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By Dr. John J. Correia and Dr. H. William Detrich, III (Eds.)

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7, 547–568. , and Crothers, D. M. (1975). Interactions of heteroaromatic compounds with nucleic acids. 1. The influence of heteroatoms and polarizability on the base specificity of intercalating ligands. Eur. J. Biochem. 54, 267–277. Myszka, D. G. (2000). Kinetic, equilibrium and thermodynamic analysis of macromolecule interactions with BIACORE. Methods Enzymol. 323, 325–332. , Santai, C. , Jain, S. , and Hud, N. V. (2004). Assembly of an antiparallel homo-adenine DNA duplex by small-molecule binding.

Measurements of Binding Interactions in Transcription Regulation A. Determine the Assembly State of the Protein(s) of Interest B. Small Ligand Binding C. Analysis of Binding Data D. Tools for Measuring Protein–Protein Interactions E. Protein–DNA Interactions V. Case Studies of Multiple Linked Equilibria in Transcription Regulatory Systems A. CRP and CytR: Combinatorial Control of Transcription in E. coli B. The Progesterone Receptor: A Nuclear Hormone Receptor References Abstract Assembly of transcriptional regulatory complexes often involves multiple binding processes and these binding processes are frequently coupled to one another.

H. (1979b). Ethidium fluorescence assays. Part I. Physicochemical studies. Nucleic Acids Res. 7, 547–568. , and Crothers, D. M. (1975). Interactions of heteroaromatic compounds with nucleic acids. 1. The influence of heteroatoms and polarizability on the base specificity of intercalating ligands. Eur. J. Biochem. 54, 267–277. Myszka, D. G. (2000). Kinetic, equilibrium and thermodynamic analysis of macromolecule interactions with BIACORE. Methods Enzymol. 323, 325–332. , Santai, C. , Jain, S. , and Hud, N.

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