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By Ramakant Sharma

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6 - colour changes from straw-yellOW to bluish-green) are commonly used as indicators. 5. NOTE:-Indicator paper strips shall always be kept in a closed glass bottle and in a dry condition. 3. 9. 8. 9 should be regarded with suspicion as indication of some diseases of the udder or of late lactation milk. 9 Clot on Boiling (COB) 1. Introduction -Clot on BOiling (COB) is used as rapid platform test for accepting milk at collection and chilling centres and the dairy plants. This is a quick test for determining the quality of milk and to find out its suitability for pasteurization, boiling etc.

4 Observe the colour of the mixture. 8) shows a yellow colour when brom thymol blue is added. 4) indicates mastitis infection. A negative test cannot be taken as evidence of absence of infection. Note: Milk from cows in advanced lactation also has an alkaline reaction and therefore gives a positive test. 1 Introduction - Milk drawn from infected quarters generally contains excessive number of leucocyte cells which can be detected by making a microscopic examination of the milk smear. 1 Compound microscope and other items as given indirect microscopic count of milk.

Of this solution mixed with 10ml. obtaining a final concentration of 1/300,000 for the dye which has been found to be satisfactory for the test. The stock solution must be stored in a sterile glass stoppered amber coloured bottle in a dark place. Fresh solution must be prepared once in two months. 4. Procedure:(i) Thoroughly mix the samples of the milk. (ii) Transfer 10ml. of each sample of milk into a test tube. (iii) Adc;! 1ml of the methylene blue solution to the milk in the test tubes and replace the cotton plugs with sterile rubber bungs using sterile forceps.

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