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Extra resources for Electrophoresis a survey of techniques and applications: Part B: Applications

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79). During the e l u t i o n of t h e dye the polysaccharides remain within the gel and can be re-stained". The actual procedure of Alcian blue s t a i n ing was l a t e r s l i g h t l y modified. Instead of t h i s dye, s t a i n i n g can a l s o be c a r r i e d out w i t h t o l u i d i n e blue ( i n water a t pH 6 - 7 ) , which i s p a r t i c u l a r l y s u i t a b l e f o r c e l l u l o s e a c e t a t e s t r i p s . The excess of t h e dye i s removed by washing with ethanol. Dudman and Bishop81 used r e a c t i v e dyes, Procion Blue 3GS and Procion Red 2BS, with which the polysaccharides a r e stained before electrophoresis so t h a t they move a s already coloured zones.

G . , f r u i t s , f r u i t j u i c e s and jams has been e x t e n s i v e l y s t u d i e d . The analyses can e a s i l y be performed w i t h o u t sample p r e - t r e a t m e n t i n t h e column-coupling system17 o r w i t h a s i m p l e e x t r a c t i o n and/or centrifugation. , serum, u r i n e , j u i c e ) can be d i r e c t l y determined T h i s column-coupling system w i l l i n c r e a s e q u a l i t a t i v e l y and t h e range o f a p p l i c a t i o n s o f i s o t a c h o o h o r e s i s , because h i g h sample l o a d s a r e p e r m i t t e d , t h e a n a l y s i s t i m e i s i n c r e a s e d n e g l i g i b l y by h i g h sample l o a d s , h i g h r a t i o s o f c o n c e n t r a t i o n s between t h e samDle comipounds a r e p e r m i t t e d and, i f necessary, d i f f e r e n t o p e r a t i n g c o n d i t i o n s can be a p p l i e d i n t h e s e p a r a t i o n and p r e s e p a r a t i o n compartments.

M i k k e r s , F. E v e r a e r t s , F. Oerlemans and C. de Bruyn, J . , 182 (1980) 317. 20 A. -G. H,jalmarsson and G. Neumann, 2 . Anal. , 290 (1978) 148. 21 T. Yagi, Y. S h i o g a i and J. Akiyama, Shimadzu, Hydron, 34, 4 (1977) 229. 22 K. K a i s e r and H. Hupf, Deut. , 75 (1979) 346. 23 K. K a i s e r and H. Hupf, Deut. , 75 (1979) 300. L. H. Pasmans, ' s Gravenhage, 1973. 25 A. Kopwillem, Acta Chem. , 27 (1973) 2426. 26 J. Dunn and R. Kemp, Protides BioZ. FZuids, 22 (1975) 727. 27 B. S j i j d i n , A.

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