Download Epithelial Transport: A guide to methods and experimental by Simon A. Lewis (auth.), Nancy K. Wills PhD, Luis Reuss MD, PDF

By Simon A. Lewis (auth.), Nancy K. Wills PhD, Luis Reuss MD, Simon A. Lewis PhD (eds.)

Building from basic ideas, the authors essentially clarify the basic position of epithelia in plasma electrolyte and water stability. Emphasis is put on experimental methods and technique. A entire thesaurus of phrases is included.

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The turnover number is about 100-200 per second. The cardiac glycoside ouabain is a very specific blocker of this pump; however, the affinity of ouabain for the a-subunit is isoform dependent. Distribution and regulation This pump is found in all vertebrate epithelia studied to date. It is generally expressed in the basolateral membrane but in the choroid plexus and the retinal pigment epithelium the pump is in the apical membrane. One mechanism of regulation, demonstrated in the lacrimal gland acinar cell, is the rapid movement of cytoplasmic vesicles into the basolateral membrane.

And Tsien, RW. (1994) Structural basis of ion channel permeation and selectivity. Curro Opin. Neurobioi. 4:313-23. , Lopes, A. B. (1994) Chloride channels along the nephron, in Chloride Channels (ed. B. 265-316. Stein, W. D. (1990) Channels Carriers, and Pumps. , San Diego, CA, 326 pp. Taglialatela, M. M. (1994) Structural correlates of K+ channel function. NIPS 9:169-73. Takumi, T. (1993) A protein with a single transmembrane domain forms an ion channel. NIPS 8:175-7. Thorens, B. (1993) Facilitated glucose transporters in epithelial cells.

In epithelial cells, secondary active transport is in most instances coupled to Na+ transport. Thus the energy stored in the Na+ electrochemical gradient (produced by the Na+ pump) is used to transport Na+ and other substrates. This sodium-coupled transport is called cotransport when Na+ moves down its electrochemical gradient and the other substrate moves in the same direction (in many instances against a net chemical or electrochemical gradient). In countertransport, the movement of Na+ down its electrochemical gradient produces the movement of the substrate in the opposite direction.

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