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Extra resources for Hepatitis E Virus
Sect F Struct Biol Cryst Commun 64(Pt 4):318–322 85. Wei S, Walsh P, Huang R, To SS (2000) 93G, a novel sporadic strain of hepatitis E virus in South China isolated by cell culture. J Med Virol 61(3):311–318 86. Wiertz EJ, Tortorella D, Bogyo M, Yu J, Mothes W, Jones TR, Rapoport TA, Ploegh HL (1996) Sec61-mediated transfer of a membrane protein from the endoplasmic reticulum to the proteasome for destruction. Nature 384(6608):432–438 87. Xing L, Li TC, Mayazaki N, Simon MN, Wall JS, Moore M, Wang CY, Takeda N, Wakita T, Miyamura T, Cheng RH (2010) Structure of hepatitis E virion-sized particle reveals an RNAdependent viral assembly pathway.
The bands 35 kDa (MeT), 38 kDa (Hel), and 36 kDa (RdRp) were identiﬁed from these expression products by using anti-MeT, anti-Hel, and anti-RdRp antibodies . Only a sole 191 kDa polyprotein was produced when the recombinant plasmid pTriEx-ORF1 is expressed in an in vitro transcription–translation system, but when this plasmid was transfected into S10-3 cells, an N-terminal product of 35 kDa and a C-terminal product of 78 kDa were detected by an immunoprecipitation assay . When expressing ORF1 in a baculovirus–insect system in the form of fusion protein His6-ORF1-Flag, a polyprotein of 192 kDa was produced, and the number of processed short fragments that reacted with anti-His and anti-Flag antibodies increased overtime.
Virus Res 161(1):47–58 3. Ansari IH, Nanda SK, Durgapal H, Agrawal S, Mohanty SK, Gupta D, Jameel S, Panda SK (2000) Cloning, sequencing, and expression of the hepatitis E virus (HEV) nonstructural open reading frame 1 (ORF1). J Med Virol 60(3):275–283 4. Billam P, Sun ZF, Meng XJ (2007) Analysis of the complete genomic sequence of an apparently avirulent strain of avian hepatitis E virus (avian HEV) identiﬁed major genetic differences compared with the prototype pathogenic strain of avian HEV.