By Mark F. Vitha(eds.)
An more and more well known analytical approach, hydrophilic interplay chromatography (HILIC) has the facility to keep and separate polar compounds which are frequently tricky to research through reversed-phase high-performance liquid chromatography (HPLC) or different analytical tools. providing a entire overview, this publication allows readers to increase a primary knowing of the way HILIC works after which observe that wisdom to increase and enforce quite a few sensible functions.
Hydrophilic interplay Chromatography starts off with discussions of HILIC retention mechanisms, desk bound stages, and normal approach improvement. This units the basis for the book's vast assurance of functions. The authors tackle particular separation demanding situations for bioanalytical, environmental, pharmaceutical, and biochemical purposes. additionally, there's a thorough dialogue of HILIC in two-dimensional chromatography.
With contributions from major analytical scientists who've broad adventure in HILIC in addition to HPLC, Hydrophilic interplay Chromatography serves as a realistic consultant for researchers, that includes:
- Detailed examples of HILIC equipment and improvement techniques
- Thorough motives of retention mechanisms and the influence of desk bound part and cellular section homes on separations
- Step-by-step information for constructing effective, delicate, and powerful HILIC tools
- References to the first literature on the finish of every bankruptcy
Hydrophilic interplay Chromatography is written for scientists who use or enhance analytical equipment for the separation of polar compounds. particularly, those researchers will detect how HILIC can be utilized to research and higher comprehend the composition of pharmaceutical, bioanalytical, biochemical, chemical, nutrition, and environmental samples.
Chapter 1 Separation Mechanisms in Hydrophilic interplay Chromatography (pages 1–41): David V. McCalley
Chapter 2 desk bound levels for Hilic (pages 43–85): Mohammed E. A. Ibrahim and Charles A. Lucy
Chapter three Hilic procedure improvement (pages 87–110): Yong Guo and Xiande Wang
Chapter four Pharmaceutical functions of Hydrophilic interplay Chromatography (pages 111–168): Bernard A. Olsen, Donald S. Risley, V. Scott Sharp, Brian W. Pack and Michelle L. Lytle
Chapter five Hydrophilic interplay Chromatography (HILIC) for Drug Discovery (pages 169–193): Alfonso Espada and Mark Strege
Chapter 6 Advances in Hydrophilic interplay Chromatography (HILIC) for Biochemical purposes (pages 195–217): Fred Rabel and Bernard A. Olsen
Chapter 7 HILIC?MS for distinctive Metabolomics and Small Molecule Bioanalysis (pages 219–238): Hien P. Nguyen, Heather D. Tippens and Kevin A. Schug
Chapter eight HILIC for nutrition, Environmental, and different purposes (pages 239–264): Michael A. Koupparis, Nikolaos C. Megoulas and Aikaterini M. Gremilogianni
Chapter nine idea and perform of Two?Dimensional Liquid Chromatography Separations related to the HILIC Mode of Separation (pages 265–305): Stephen R. Groskreutz and Dwight R. Stoll
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Additional resources for Hydrophilic Interaction Chromatography: A Guide for Practitioners
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Mixed-mode ionexchangers and their comparative chromatographic characterization in reversedphase and hydrophilic interaction chromatography elution modes. J. Sep. Sci. 2008; 31: 2572–2588. 9. Wu J, Bicker W, Lindner W. Separation properties of novel and commercial polar stationary phases in hydrophilic interaction and reversed-phase liquid chromatography mode. J. Sep. Sci. 2008; 31: 1492–1503. references 39 10. Alpert AJ. Hydrophilic interaction chromatography for the separation of peptides, nucleic acids and other polar compounds.
Chromatogr. A 2011; 1218: 6630–6638. 31. Hemström P, Irgum K. Hydrophilic interaction chromatography. J. Sep. Sci. 2006; 29: 1784–1821. 32. Orth P, Engelhardt H. Separation of sugars on chemically modified silica-gel. Chromatographia 1982; 15: 91–96. 33. Guo Y, Huang A. A HILIC method for the analysis of tromethamine as the counter ion in an investigational pharmaceutical salt. J. Pharm. Biomed. Anal. 2003; 31: 1191–1201. 34. Li R, Zhang Y, Lee CC, Liu LM, Huang YP. Hydrophilic interaction chromatography separation mechanisms of tetracyclines on amino-bonded silica column.