By Elmar Heinzle, Matthias Reuss (auth.), Elmar Heinzle, Matthias Reuss (eds.)
This booklet relies at the contributions to the IFAC-Workshop "Mass Spectro metry in Biotechnological approach research and keep watch over" held in Graz, Austria from 23 to 24 October 1986. the belief to prepare this workshop and additional to organize those complaints was once encouraged by way of the next proof. Biotechnological tactics urgently want greater online instrumentation. Mass spectrometry (MS) bargains a good capability to in particular examine gases and unstable compounds. it's, although, thought of that this strength via a ways isn't exhausted. the most reason behind this can be that MS frequently nonetheless is taken into account to be a truly dear strategy requiring the everlasting recognition of a MS specialist. moreover tools haven't but been constructed to a person pleasant nation. online MS-methods can be found to a undeniable volume, yet want extra improvement. To stimulate such improvement an interdisciplinary attempt is critical. wishes of commercial and collage clients and event of physicists and software brands need to be introduced right into a optimistically fruitful dialogue. An introductory article describes the bioprocess heritage together with a short precis of the state-of-the-art in bioprocess sensor and parameter estimation improvement, and the capability MS deals for bioprocess tracking. within the first bankruptcy on "Instrumentation and fuel research" a basic assessment on a few advancements in MS-instrumentation is given first and foremost by way of Schmid. Then the almost immediately to be had instrumentation for bioprocess tracking is mentioned by means of tool brands (Winter; Schaefer and Schultis; Bartman).
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Extra info for Mass Spectrometry in Biotechnological Process Analysis and Control
HU U ... I·ln~ :adrUPOle Analyser ~: ~. : - • 00 • "g"q; + tttt Ion Source MASS-SCANNING BY ADJUSTMENT OF RF AND DC ELECTRIC FIELDS ADVANTAGES DISADVANTAGES SMALLER LESS ACCURACY CHEAPER LESS PRECISION FASTER ABUNDANCE SENSITIVITY CONTAMINATION EFFECTS - QUADRUPOLE SYSTEM MAY BE APPROPRIATE WHEN MAKING A 'SIMPLE' ANALYSIS AND THE HIGHEST PRECISIONS AND ACCURACIES ARE NOT DEMANDED Figure 3: Quadrupole analysers 22 Magentic Sector Analysers Magnetic Sector Analysers depend upon a different principle - the deflection caused to an ion beam by a magnetic, rather than electric field as in the quadrupole.
The sample from the reactor headspace is introduced into the ionizer where it is transformed to a positively charged species by collision with an electron. This collision can produce both parent ions, having a mass-to-charge (m/z) ratio equal to the compound's molecular weight, and fragment ions. The cloud of positively charged sample ions is collimated by a series of lenses for introduction into the quadrupole mass filter. The quadrupole is a set of four cylindrical rods held in a square array to which an RF and DC potential are applied.
The possibility of linking other sensors to the K5-32 system; 7. Control of a bioreactor, using the K5-32 system; 8. Practical experience gained by using the combination KS-32 and MGA-I200: Many aerobic fermentation processes are very complex and difficult to optimize. Therefore, a better understanding of a typical fermentation process is often reached by observing the way in which the microbe responds - in terms of what it produces - under different environmental conditions. On-line measurements are particularly valuable because the information can be sent directly to a computer.