By Morris Kates, Arnisa Kuksis
This publication represents the complaints of a satellite tv for pc Symposium of the XIth overseas Congress of Biochemistry on "Control of Membrane Fluidity" which used to be hung on July 7, 1979 on the Charles H. most sensible Institute, college of Toronto, Toronto, Canada. The assembly used to be prepared by means of M. Kates and A. Kuksis and used to be supported by way of the foreign Congress of Biochemistry. the aim of the assembly was once to study contemporary development in lots of various components of research touching on the position of lipids within the structural and useful estate of the mobile membrane generally known as fluidity. the purpose used to be to stress the standards controlling membrane fluidity as studied in applicable in vitro and in vivo experiments. The Symposium incorporated invited assessment papers and brief papers provided via discussants. In assem· bling the e-book no contrast has been made among the 2 kinds of shows, nor has any importance been connected to the chronological order in their presentation within the Symposium. therefore it used to be attainable to supply a way more coherent and non-stop presentation than that on hand on the meeting.
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Extra info for Membrane Fluidity: Biophysical Techniques and Cellular Regulation
1968). It may not always be applied to complex lipids because of difficulties in vaporization. HPLC employing a flame ionization detector was successfully applied to determination of neutral lipids and Abbreviations: TLC,thin-layer chromatography; HPTLC,highperformance thin-layer chromatography; HPLC,high-performance liquid chromatography; TL,total lipids; NL,neutral lipids; PL,phospholipids; CE,cholesteryl ester; FC,free cholesterol; TG,triacylglycerol; FA, free fatty acid; PE,phosphatidylethanolamine; PC,phosphatidylcholine; LPC,lysophosphatidylcholine; 8M,sphingomyelin; P8,phosphatidylserine.
K. Saito and M. Kates (1978). SEectrometry 5, 87. Biomed. M. (1969). In E. M. ), Structural and Functional Aspects of LiEoEroteins in Living Systems. Academic Press, New York. p. 425. , Dielmann, H. Husmann and F. Waeke (1976). J. Chromatogr. 122, 55. A. D. Collins (1975). Acta. 409, 104. Biochim. Biophys. A. D. Collins (1975). Acta. 409, 116. Biochim. Biophys. P. (1972). J. ), Blood Lipids and Lil20Eroteins. Wiley-Interscience, New York. 47l. L. (1973). Arch. Biochem. BioEhys. 154, 47. Sundler, R.
Biophys. , A. Kuksis, L. J. Myher (1977). Lipids 12, 529. , 1,2-da1ky1, 1-a1kenyl-2-acy1, 1-a1kyl-2-acy1 and 1,2-diacy1 glycerols as TMS or TBDMS derivatives were separated and identified by monitoring three kinds of fragment ions, 1) M-15 or M-57 for determining molecular weight; 2) RCO + 74, R + 130 and RCH=CH + 56 for acyl, alkyl and a1keny1 residues, respectively, and 3) base peaks corresponding to TMS-g1ycero1 (m/e 129,130) or O-TBDMS (m/e 131). By this method, chronological changes in molecular species of choline glycerophospho1ipids of fetal and adult rat liver and brain were studied.