By Xueji Zhang, Haifeng Dong, Yaping Tian
This ebook summarizes microRNA (miRNA) biology in a number of pathological approaches, emphasizing the numerous capability functions of miRNA in diagnostics and prognostics, in addition to novel drug ambitions. the normal innovations used for miRNA detection together with commonplace PCR, Northern blotting, microarray and clone equipment are addressed. fresh rising suggestions in miRNA detection and quantification with stronger flexibility and flexibility, resembling novel molecular organic concepts and locked nucleic acid (LNA) converted probes, in addition to nanotechnology-based ways, also are integrated. The booklet additionally highlights the newest advances in clinical-related miRNA detection equipment in dwelling cells, circulating blood and tissue, akin to in situ hybridization (ISH) and molecular imaging recommendations, that are priceless to explain the biogenesis and organic functionality of miRNAs in vivo. eventually, the respective benefits and disadvantages of assorted detection innovations during this fast-moving box are mentioned, besides the demanding situations and promising new directions.
This booklet deals a precious source for analytical chemists, biologists and physicians focused on miRNA research.
Dr. Xueji Zhang and Dr. Haifeng Dong are Professors on the institution of Chemistry & organic Engineering, college of technology & expertise Beijing (USTB), China.
Dr. Yaping Tian is a Professor on the division of medical Biochemistry, chinese language PLA basic health facility and army clinical university, China.
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Extra resources for MicroRNA Detection and Pathological Functions
Neely LA, Patel S, Garver J, Gallo M, Hackett M, McLaughlin S, Nadel M, Harris J, Gullans S, Rooke J (2006) A single-molecule method for the quantitation of microRNA gene expression. Nelson PT, Baldwin DA, Scearce LM, Oberholtzer JC, Tobias JW, Mourelatos Z (2004) Microarray-based, high-throughput gene expression profiling of microRNAs. Sato F, Tsuchiya S, Terasawa K, Tsujimoto G (2009) Intra-platform repeatability and interplatform comparability of microRNA microarray technology. Reinhart BJ, Slack FJ, Basson M, Pasquinelli AE, Bettinger JC, Rougvie AE, Horvitz HR, Ruvkun G (2000) The 21-nucleotide let-7 RNA regulates developmental timing in Caenorhabditis elegans.
Tang X, Gal J, Zhuang X, Wang W, Zhu H, Tang G (2007) A simple array platform for microRNA analysis and its application in mouse tissues. Rarnkissoon SH, Mainwaring LA, Sloand EM, Young NS, Kajigaya S (2006) Nonisotopic detection of microRNA using digoxigenin labeled RNA probes. Pall GS, Codony-Servat C, Byrne J, Ritchie L, Hamilton A (2007) Carbodiimide-mediated cross-linking of RNA to nylon membranes improves the detection of siRNA, miRNA and piRNA by northern blot. Varallyay E, Burgyan J, Havelda Z (2008) MicroRNA detection by northern blotting using locked nucleic acid probes.
This strategy gave improved sensitivity with an estimated LoD of 6 fm miRNA. In the other two sensors, immobilization of HRP–streptavidin conjugates is replaced by target miRNA hybridizationdriven unmasking of DNA sequences able to fold into G-quadruplexes. Addition of hemin results in supramolecular complexes with peroxidase-like catalytic activity allowing miRNA quantification. Although similar in design, these two approaches considerably differ in terms of sensitivity with LoD of about 6 pm  and approximately 6 fm  respectively.