Download Microscale Diagnostic Techniques by M.L. Gardel, M.T. Valentine, D.A. Weitz (auth.), Prof. PDF

By M.L. Gardel, M.T. Valentine, D.A. Weitz (auth.), Prof. Kenneth S. Breuer (eds.)

Microscale Diagnostic Techniques highlights the main cutting edge and robust advancements in microscale diagnostics. It presents a source for scientists and researchers drawn to studying in regards to the strategies themselves, together with their features and obstacles. The fields of Micro- and Nanotechnology have emerged during the last decade as a massive concentration of contemporary clinical and engineering learn and know-how. pushed by means of advances in microfabrication, the research, manipulation and engineering of platforms characterised by means of micrometer and, extra lately, nanometer scales became common all through all technical disciplines. With those advancements, a wholly new choice of experimental concepts has been constructed to discover and signify such systems.

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Microrheology 33 Fig. 6. The comparison of one- and two-particle microrheology to bulk measurements in a guar solution seen by Crocker et al. (Crocker, et al. 25% weight guar solution. Due to the heterogeneous nature of the medium, the curves differ by a factor of two. The solid line is a smooth fit to the data used for calculating rheology. (b) The elastic (filled circles) and loss (open circles) moduli calculated using the distinct MSD showing a crossover at high frequencies are in good agreement with the mechanical bulk measurement (solid curves).

A second light scattering technique, Diffusing Wave Spectroscopy (DWS), allows measurements of multiple scattering media and extends the accessible frequency range to much higher frequencies (Pine, et al. 1988, Weitz and Pine 1993). The experimental set-up is similar to that of the single-scattering experiment; however, in this case, a laser beam impinges on an opaque sample and the light is scattered multiple times before exiting. The diffusion equation is used to describe the propagation of light through the sample.

For DLS measurements, only samples that are nearly transparent may be used in order to insure that each incoming photon is scattered only once before reaching the detector. For fluids or low contrast polymer solutions, a small amount of col-5 -6 loidal particles are added to a final volume fraction of 10 – 10 . Typically, the scattering signal from the colloidal particles is significantly larger than the scattering from the solvent. For cloudy and opaque samples, single light scattering measurements are not possible, and the Diffusing Wave Spectroscopy (DWS) method should be used.

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