By Andre P. De Leenheer, Willy Lambert
3rd variation collects and examines the large proliferation of data on chromatographic research of fats and water soluble supplements during the last decade. widely describes pattern practise and ultimate size.
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Extra info for Modern chromatographic analysis of vitamins
Poor et al. (224) used HPLC analysis to study the accumulation of α- and β-carotenes in serum and tissues of preruminant calves fed raw and steamed carrot slurries. Serum and various tissues of ferrets following a dose with β-carotene were extracted with a mixture of chloroform-methanol (2: 1) followed by extraction with hexane; the extracts were analyzed by HPLC (225–229). Rat Hepatoma Cells. Cis-trans conversion of β-carotene in FU-5 rat hepatoma cells was studied by HPLC analysis of cell extracts using a 5-µm Suplex PKB 100 column and a mobile phase of methanol/dichloromethane (97: 3) (230).
Column chromatography on DEAE-Toyopearl with acetone followed by another column chromatography on silica gel with hexane, followed by HPLC on a C18 cartridge with methanol, was used to isolate and purify a novel carotenoid ester, loroxanthin dodecenoate, from Pyramimonas parkeae and from a chlorarachniophycean alga (262). Braunixanthins 1 and 2 were separated and characterized as new carotenoids in the green microalga Botryococcus braunii by column chromatography on silica gel and ODS, followed by HPLC on a Develosil 60-3 column 49 Copyright 2000 by Marcel Dekker, Inc.
A C18 column with an isocratic mobile phase of acetonitrile : water :methanol : n-butyl alcohol: ammonium acetate :acetic acid was used. Lower limits of detection were on the order of 80 to 170 nM (25 to 50 ng/mL) for retinoic acid and its metabolites, using absorbance detection; an anthracenyl retinoid was used as internal standard. A similar extraction procedure was used by Guiso et al. to analyze retinoic acid and its metabolites in human plasma; proteins were precipitated with acetonitrile, 37 Copyright 2000 by Marcel Dekker, Inc.