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Urine samples have also been analyzed by Shum et al. (1992) after separation with ion-pair liquid chromatography followed by ICP-MS detection with direct injection nebulization. A 24 h urine specimen was analyzed by this method and Hg 2§ methylHg§ and ethylHg§ species were found. Other Metals Trialkyltins are the organotins having the greatest biocidal activity in mammalians. A purge and trap flame photometric gas chromatographic technique for speciation of trace organotin and organosulphur compounds in human urine standard reference material was reported by Olson et al.

L. CERVERA, and A. MORALES-RUBIO bound to transferrin, indicating both elements do not dissociate from transferrin during gradient elution. 0%. Lead The speciation of lead has attracted great deal of interest particularly with respect to organolead compounds. A high-resolution GC-ETAAS method for the determination of trimethyllead salts in human blood samples has been described by Nygren and Nilsson (1987). The method involves several steps including complexation with sodium diethyldithiocarbamate (pH 9), extraction with pentane, evaporation, and butylation by using a Grignard reagent in tetrahydrofuran.

Investigations of protein-bound trace metals in human milk before and after the birth of a baby showed marked differences in zinc and iron bound to citrate. An increase of citrate concentration by a factor of 25 + 3 takes place after birth at the first day of milk secretion. 5% Eu were recovered in pectin when fresh pasteurized skimmed cow's milk spiked with the respective radionuclides (85Sr, 137Cs,and 152Eu) was shaken with a 4% aqueous solution of apple pectin at an initial milk/pectin volume ratio of 7:3.

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